The Nigerian Under 23 men side got its first defeat at the ongoing Olympic games when Colombian team trashed the Dream Team two nil in their group B third match.
The match played at the Arena Corinthians in Sao Paulo saw the Samson Siasia tutored side make five changes to their regular starting line-up, as they had qualified for the quarter final after winning their first two games against Japan and Sweden.
The South Americans broke the deadlock on the 4th minute through their captain, Teofilo Gutierrez as he pounced on a pass from Diego Pabon to knock it past Akpeyi.
Nigeria struggled to level the scores as Siasia, made series of changes in the second half but those were not enough to pull a comeback.
Colombia sealed their win on the 63rd minute after goalkeeper, Akpeyi brought down Harold Preciado inside the box, Pabon slotted home from the penalty spot.
Nigeria, topping the group with six points, will challenge Denmark on Saturday as the host, Brazil tangles with Colombia, South Korea face Honduras and Portugal battles Germany in the quarter final.